cd122 antibody  (Elabscience Biotechnology)

Journal: Frontiers in Molecular Biosciences

Article Title: JAK-centric explainable few-shot gene-expression diagnosis framework for alopecia via MultiPLIER priors and relation-style set-to-set comparison

doi: 10.3389/fmolb.2025.1753206

Figure Lengend Snippet: RT-qPCR validation of the IL2RB/IL2RG–EOMES–GZMA cytotoxic/JAK axis in alopecia areata (AA). Relative mRNA levels of EOMES, GZMA, IL2RB, and IL2RG are significantly elevated in AA lesional scalp compared with healthy controls, whereas androgenetic alopecia (AGA) samples are comparable to controls, indicating that this cytotoxic/JAK module is transcriptionally active in AA but largely quiescent in AGA. Data are shown as mean ± SD. Statistical annotations: ** p < 0.01 vs. control; n.s., not significant (AGA vs. control).

Article Snippet: Equal amounts of protein were resolved by SDS–PAGE and transferred to PVDF membranes at 200 mA for 2 h. Membranes were blocked in 5% non-fat milk at room temperature for 2 h and incubated overnight at 4 ° C with primary antibodies against β -actin (1:4000; 20536-1-AP, Proteintech, China), EOMES (1:5000; 83945-5-RR, Proteintech, China), GZMA (1:500; 11288-1-AP, Proteintech, China), IL2RB (1:5000; 13602-1-AP, Proteintech, China), and IL2RG (1:500; 11409-1-AP, Proteintech, China).

Techniques: Quantitative RT-PCR, Biomarker Discovery, Control

Journal: Frontiers in Molecular Biosciences

Article Title: JAK-centric explainable few-shot gene-expression diagnosis framework for alopecia via MultiPLIER priors and relation-style set-to-set comparison

doi: 10.3389/fmolb.2025.1753206

Figure Lengend Snippet: GSEA plots for key genes. (a) EOMES. (b) GZMA. (c) IL2RB. (d) IL2RG.

Article Snippet: Equal amounts of protein were resolved by SDS–PAGE and transferred to PVDF membranes at 200 mA for 2 h. Membranes were blocked in 5% non-fat milk at room temperature for 2 h and incubated overnight at 4 ° C with primary antibodies against β -actin (1:4000; 20536-1-AP, Proteintech, China), EOMES (1:5000; 83945-5-RR, Proteintech, China), GZMA (1:500; 11288-1-AP, Proteintech, China), IL2RB (1:5000; 13602-1-AP, Proteintech, China), and IL2RG (1:500; 11409-1-AP, Proteintech, China).

Techniques:

Journal: Frontiers in Molecular Biosciences

Article Title: JAK-centric explainable few-shot gene-expression diagnosis framework for alopecia via MultiPLIER priors and relation-style set-to-set comparison

doi: 10.3389/fmolb.2025.1753206

Figure Lengend Snippet: Western blot validation of the IL2RB/IL2RG–EOMES–GZMA cytotoxic/JAK axis at the protein level. Protein expression of EOMES, GZMA, IL2RB, and IL2RG is markedly upregulated in AA lesional scalp compared with healthy controls, concordant with the RT-qPCR results, whereas AGA-affected scalp shows no significant difference relative to controls. Representative immunoblots and densitometric quantification (normalized to β -actin) are shown. Data are presented as mean ± SD. Statistical annotations: ** p < 0.01 vs. control; n.s., not significant (AGA vs. control).

Article Snippet: Equal amounts of protein were resolved by SDS–PAGE and transferred to PVDF membranes at 200 mA for 2 h. Membranes were blocked in 5% non-fat milk at room temperature for 2 h and incubated overnight at 4 ° C with primary antibodies against β -actin (1:4000; 20536-1-AP, Proteintech, China), EOMES (1:5000; 83945-5-RR, Proteintech, China), GZMA (1:500; 11288-1-AP, Proteintech, China), IL2RB (1:5000; 13602-1-AP, Proteintech, China), and IL2RG (1:500; 11409-1-AP, Proteintech, China).

Techniques: Western Blot, Biomarker Discovery, Expressing, Quantitative RT-PCR, Control

Journal: Experimental & Molecular Medicine

Article Title: Discovery of intrahepatic CD103 + cDC1/CD8 + T RM protective immune axis against acetaminophen-induced acute liver injury

doi: 10.1038/s12276-025-01565-3

Figure Lengend Snippet: a , b The frequency of hepatic CD69 + T RM cells in WT APAP mice ( n = 3 per group per time point) ( a ) and in WT and Batf3-KO mice 24 h after APAP administration ( n = 3 per group) ( b ). c The frequency of Ki67 + cells in hepatic CD69 + KLRG1 − CD44 + CD8 + T RM cells in APAP mice. n = 4 per group. d The population of adoptively transferred OT-I T cells and of OT-I T RM cells in the liver of recipient mice after APAP administration. n = 3 per group. e The frequency of CD69 + T RM cells was assessed when APAP mice were pretreated with blocking antibodies against IL-12, IL-15 and IL-18. n = 4 per group. f The ALT level was assessed in APAP mice pretreated with IL-15 blocking antibodies (α-CD122). n = 3 per group per time point. g The frequency of IL-15Rα + cells in the hepatic DC1s and DC2s of APAP mice. n = 3 per group. h The frequency of T RM cells in hepatic CD8 + T cells when CD8 + T cells were cultured in the presence of IL-15. n = 4 per group. i The frequency of T RM cells among CD8 + T cells when hepatic CD8 + T cells were cocultured with LPS-stimulated hepatic DC1s of APAP mice in the presence of IL-15 blocking antibodies. n = 4 per group. Ordinary one-way ANOVA with Dunnett for posttest in a and e , unpaired two-tailed Student’s t -test in b – d and g – i and two-way ANOVA with Bonferroni for posttest in f were used to measure significance. The error bars indicate mean ± s.e.m. ns, not significant. * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: For IL-12, IL-15, IL-18 and CCL2 neutralizing, mice were i.p. injected with 200 μg per mouse of anti-IL-12 antibody (clone R1-5D9), anti-CD122 (IL-15Rβ) antibody (clone TM-Beta1), anti-IL-18 antibody (clone YIGIF74-1G7) and anti-CCL2 antibody (clone 2H5) (all from BioXCell).

Techniques: Blocking Assay, Cell Culture, Two Tailed Test

Journal: Experimental & Molecular Medicine

Article Title: Discovery of intrahepatic CD103 + cDC1/CD8 + T RM protective immune axis against acetaminophen-induced acute liver injury

doi: 10.1038/s12276-025-01565-3

Figure Lengend Snippet: a Based on the public single-cell RNA sequencing dataset from liver tissues of healthy donors and patients with APAP-ALI ( GSE223581 ), the relative expression levels of IL-15 were assessed between APAP patients and healthy donors, and the correlation between IL-15 level and CD69 + T RM population among the CD8 + T cells was evaluated. b Using the same dataset, the frequency of CD14⁺ monocytes among total liver immune cells (left) and the populaton of FCGR3A⁺ inflammatory monocytes among the CD14 + monocytes in the liver (right) were assessed between healthy donors and patients with APAP-ALI. For inflammatory monocytes, the ratio of FCGR3A >0.1 was assessed in the CD14 >0.1 population of monocytes. c The mRNA levels of IL-15 / IL-15RA were assessed by quantitative real-time PCR in LPS-activated hepatic DC1s isolated from noncancerous healthy tissues of resected liver specimens obtained from patients with HCC and other liver diseases. n = 3 per group. d , e The frequency of human hepatic CD8 + T RM cells when hepatic CD8 + T cells were cocultured with LPS-treated hepatic DC1s ( d ), in the presence of IL-15 blocking antibodies (α-CD122) ( e ). f The human hepatic inflammatory monocytes were cocultured with human hepatic T RM cells at a different ratio, and the frequency of annexin V + monocytes was assessed. n = 4 per group. Unpaired two-tailed Student’s t -test in a and b , multiple unpaired t -test in c and ordinary one-way ANOVA with Dunnett for the posttest f were used to measure significance. The error bars indicate mean ± s.e.m. ns, not significant. * P < 0.05.

Article Snippet: For IL-12, IL-15, IL-18 and CCL2 neutralizing, mice were i.p. injected with 200 μg per mouse of anti-IL-12 antibody (clone R1-5D9), anti-CD122 (IL-15Rβ) antibody (clone TM-Beta1), anti-IL-18 antibody (clone YIGIF74-1G7) and anti-CCL2 antibody (clone 2H5) (all from BioXCell).

Techniques: RNA Sequencing, Expressing, Real-time Polymerase Chain Reaction, Isolation, Blocking Assay, Two Tailed Test

Journal: Molecular Medicine Reports

Article Title: Expression, immunogenicity and clinical significance analysis of thyroid-stimulating hormone receptor fusion proteins

doi: 10.3892/mmr.2025.13639

Figure Lengend Snippet: Changes of specific regulatory T cell subsets in mice after immunization. (A) Flow cytometry analysis of changes in CD4 + CD25 + T cells in the hTSHR289, hTSHR290 and hTSHR410 groups after immunization. (B) Flow cytometry analysis of changes in CD8 + CD122 + T cells in the hTSHR289, hTSHR290 and hTSHR410 groups after immunization. *P<0.05 (One-way ANOVA with Tukey's post hoc test. hTSHR, human thyroid-stimulating hormone receptor.

Article Snippet: The analyte detectors were as follows: CD3 + antibody (cat. no. 565643; Becton, Dickinson and Company), CD4 + antibody (cat. no. F21004A02; Multi Sciences Biotech), CD8 + antibody (cat. no. F2100801; Multi Sciences Biotech), CD25 antibody (cat. no. E-AB-F1102C; Wuhan Elabscience Biotechnology Co., Ltd.) and CD122 antibody (cat. no. E-AB-F1029D; Wuhan Elabscience Biotechnology Co., Ltd.).

Techniques: Flow Cytometry

Journal: Cell reports

Article Title: Mapping the genetic landscape establishing a tumor immune microenvironment favorable for anti-PD-1 response

doi: 10.1016/j.celrep.2025.115698

Figure Lengend Snippet: (A) Cxcl9/Spp1 ratio in MC38 bulk RNA-seq samples from responder and non-responder strain tumors. Boxes show first quartile, median, and third quartile, while whiskers extend to a maximum of 1.5 times the interquartile range. Wilcoxon test, ** p < 0.01, * p < 0.05; n.s., not significant. (B) Schematic overview of the blocking antibody dosing protocol and strategy for assessing tumors before macroscopic changes in size. (C) Cxcl9/Spp1 ratio computed using RNA-seq gene expression profiling of CC75 F1 MC38 tumors collected as in (B). Boxes show first quartile, median, and third quartile, while whiskers extend to a maximum of 1.5 times the interquartile range. Wilcoxon test, **** p < 0.0001, *** p < 0.001, ** p < 0.01, * p < 0.05; n.s., not significant. (D) Principal-component (PC) plot showing the positioning of each group of samples within the first two PCs. CC75 ISO is the tumor in a responding genetic cross treated with isotype control antibodies, whereas CC80 ISO is the tumor in a non-responding genetic cross treated similarly. Principal-component analysis was performed using batch-corrected RNA-seq profiles with error bars showing +/− 1 standard error within each group. (E) Heatmap of enriched immune-related pathways among the experimental groups profiled in this blocking antibody experiment showing reversal of immune signatures. Pathway activity was computed based on leading edge genes identified by gene set enrichment analysis and constituted the mean of standardized (per-gene, across all samples) gene expression. (F) Model describing the effects of IL2RB and GM-CSF blockade on the MC38 TIME and response to aPD1. See also and .

Article Snippet: InVivo MAb anti-mouse CD122 (IL-2Rβ) (clone TM-Beta 1) , Bio X Cell , CAT#BE0298; RRID:AB_2687820.

Techniques: RNA Sequencing, Blocking Assay, Gene Expression, Control, Activity Assay